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Biophysics: Tools and Techniques - ISBN 9781498702430

Biophysics: Tools and Techniques

ISBN 9781498702430

Autor: Mark C. Leake

Wydawca: CRC Press

Dostępność: Wysyłka w ciągu 2-3 dni

Cena: 269,85 zł






Mark C. Leake



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An Up-to-Date Toolbox for Probing Biology Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes students from the physical sciences with essential biological knowledge. The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism. Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.

Mark C. Leake is the chair of biological physics and the director of the Biological Physical Sciences Institute (BPSI) at the University of York. He heads an interdisciplinary research team in the field of single-molecule biophysics using cutting-edge biophotonics and state-of-the-art genetics. His work is highly cited, and he has won many fellowships and prizes.

Introduction The motivation for biophysics What do we mean by a "toolbox?" Makeup of the subsequent chapters in this book Once more, unto the breach Summary points Questions References Orientation for the bio-curious Introduction: the material stuff of life Architecture of organisms, tissues and cells, and the bits in between The chemicals that make cells work Cell processes Physical quantities in biology Summary points Questions References Making light work in biology Introduction Basic UV-VIS-IR absorption, emission, and elastic light scattering methods Light microscopy basics Non-fluorescence microscopy Fluorescence microscopy: the basics Basic fluorescence microscopy illumination modes Summary points Questions References Making light work harder in biology Introduction Super-resolution microscopy Forster resonance energy transfer (FRET) Fluorescence correlation spectroscopy (FCS) Light microscopy of deep samples Advanced biophysical techniques using elastic light scattering Tools using the inelastic scattering of light Summary points Questions References Detection and imaging tools that use non-optical waves Introduction Electron microscopy (EM) X-ray tools NMR and other radio frequency and microwave resonance spectroscopies Tools that use gamma rays, radioisotope decay, and neutrons Summary points Questions References Forces Introduction Rheology and hydrodynamics tools Optical force tools Magnetic force methods Scanning probe microscopy and force spectroscopy Electrical force tools Tools to mechanically probe cells and tissues Summary points Questions References Complementary experimental tools Introduction Bioconjugation Model organisms Molecular cloning Making crystals High-throughput techniques Characterizing physical properties of biological samples in bulk Biomedical physics tools Summary points Questions References Theoretical biophysics Introduction Molecular simulation methods Mechanics of biopolymers Reaction, diffusion, and flow Advanced in silico analysis tools Rigid-body mechanics Summary points Questions References Emerging biophysics techniques Introduction Systems biology: "systems biophysics" Synthetic biology and bionanotechnology Personalizing healthcare Extending length and time scales to quantum and ecological biophysics Summary points Questions References Index


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